An HPLC setup has an injection volume of 0.412 mL, a detector volume of 0.378 mL, and contains 29.0 cm of tubing which has a diameter of 0.080 cm. | Cheap Nursing Papers

An HPLC setup has an injection volume of 0.412 mL, a detector volume of 0.378 mL, and contains 29.0 cm of tubing which has a diameter of 0.080 cm.

An HPLC setup has an injection volume of 0.412 mL, a detector volume of 0.378 mL, and contains 29.0 cm of tubing which has a diameter of 0.080 cm. The baseline width, wobs, of a band eluting from the column at a flow rate of 1.57 ml/min is observed to be 0.822 min. Assuming a diffusion coefficient of 8.0 × 10-9 m2/s, calculate the baseline width of the band, in minutes, if the column was the only source of broadening.

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