An HPLC setup has an injection volume of 0.412 mL, a detector volume of 0.378 mL, and contains 29.0 cm of tubing which has a diameter of 0.080 cm. The baseline width, wobs, of a band eluting from the column at a flow rate of 1.57 ml/min is observed to be 0.822 min. Assuming a diffusion coefficient of 8.0 × 10-9 m2/s, calculate the baseline width of the band, in minutes, if the column was the only source of broadening.
Hi there! Click one of our representatives below and we will get back to you as soon as possible.