Laboratory 5 – Mendelian Genetics – Principle of Independent Assortment lab and Principles of DNA Sequencing

Principle of Independent Assortment: In this section of today’s lab, we will conduct two exercises related to Mendel’s Principle of Independent Assortment (Investigation 1):

Principles of DNA Sequencing:

Exercise 2. The purpose of this exercise is to provide detailed instruction on the practice of DNA sequencing. You will be provided an actual autoradiograph (an exposed sheet of x-ray film) of a DNA sequencing gel for analysis. The sequence deduced from the autoradiogram will differ from a wild type sequence by a single nucleotide(s). Refer to the handout provided on Blackboard. This difference represents an actual mutation in the DNA molecule. You are expected to identify the location(s) of the mutated nucleotide(s). You can also work in groups to read the sequence and compare results to assure accuracy. Make sure you read the handout because you will see this information again on your midterm. (25 points)

Part 1 does not require a formal lab report. Just give your answers and the results of the cross. For part 2, a type written lab report is needed. Use the handout to help you put together your report. You MUST have a cover page. Total = 50 points

Read the lab report writing aid, then write a lab report, must meet all requirements.