1.    Transcribe the following DNA sequence:

Hb A:

5′ AGT AAC GGC AGA CTT CTC CTC AGG AGT CAG GTG CAC CAT 3′

2.    Translate your new RNA sequence using the genetic code. Remember that when determining your amino acid sequence, the RNA sequence is read from 5′ to 3′.

Note: the first amino acid will be Met.

3.    Transcribe the following DNA sequence.

Hb S: 

5′ AGT AAC GGC AGA CTT CTC CAC AGG AGT CAG GTG CAC CAT 3′

4.    Translate your Hb S transcript.

Note: the first amino acid will be Met.

Post-Lab Questions