2. Introduction: general background information about enzymes and specific information about your chosen enzyme, the question(s) that you are asking and a clear hypothesis for your experiment (20 points).
3. Provide a detailed description of the materials and methods used to conduct the experiment. (expeirment and results are below) Identify control and experimental samples, as well as independent and dependent variables. Also include the methods used for data collection and analysis (20 points).
5. Use your knowledge of enzymes and pH to interpret and discuss your results. It may be necessary for you to refer to the OLI course modules, lab manual and/or use additional information resources. What effect does the acidic treatment have on enzyme activity? Did you get the expected results? Explain. (20 points)
6. State a specific and accurate conclusion. Is your hypothesis supported by the results? Looking back, how could you have improved your experiment? (10 points)
7. Include a list of references to all information sources used in APA format (5 points).
Experiment & results
The effect of vinegar on catalase’s yeast activity.
Hypothesis. The enzymatic activity of the catalase’s yeast will be reduced by the addition of vinegar. The more vinegar that we add, the pH level will we be reduced and the activity will be lower.
Materials and reagents: pH paper, hydrogen peroxide solution (3%), plastic beakers or cups, (250mL or more) vinegar (acetic acid 5%), sugar, yeast, marker for labeling of beakers, tweezers, scissors, two spoons (for 1 tablespoon and ½ tablespoon measure) cylinder, chronometer, paper towel, filter paper, mortar and pestle.
Procedure:
In different plastic beakers or cups, prepare 200mL of vinegar solution using the following proportions detailed in the table. (Don’t forget to label the beakers as 0%, 1%, 5%, 10%, 50%, 100%)
Vinegar (mL) |
Water (mL) |
Concentration (%) |
0 |
200 |
0 |
2 |
198 |
1 |
10 |
190 |
5 |
20 |
180 |
10 |
100 |
100 |
50 |
200 |
0 |
100 |
Results of experiment
0 – 5.58 seconds
1% – 13.50 seconds
5% – 14.15 seconds
10% – 20.75 seconds
50% – 1m05s20 seconds
100% – 20 minutes later filter paper still stayed submerged in the solution
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