Identify the DNA sequence (put the whole sequence in your homework submission), locate the start codon, total number of coding amino acids and translate the first 20 amino acids. (Hint: Use NCBI database, gene ID: 945006) Design two primers (state melting temperatures) to amplify the full length of this gene using PCR. Specify the PCR conditions you would use for the amplification, assuming you use Taq polymerase (elongation rate: 1000bp/min).
Hi there! Click one of our representatives below and we will get back to you as soon as possible.