Biology 102/103
Lab 5: Meiosis
INSTRUCTIONS:

On your
own and without assistance, complete this Lab 5Answer Sheet electronically and
submit it via the Assignments Folder by the date listed intheCourse Schedule
(under Syllabus).
To
conduct your laboratory exercises, use the Laboratory Manual located under
Course Content. Read the introduction and the directions for each
exercise/experiment carefully before completing the exercises/experiments and
answering the questions.
Save your
Lab 5Answer Sheet in the following format:
LastName_Lab5 (e.g., Smith_Lab5).
You
should submit your document as a Word (.doc or .docx) or Rich Text Format
(.rtf) file for best compatibility.
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Pre-Lab Questions
1. Compare and contrast mitosis and meiosis.

2. What
major event occurs during interphase?

Experiment 1: Following Chromosomal DNA Movement through
Meiosis
Data Tables and Post-Lab Assessment
Trial 1 – Meiotic Division Without Crossing Over Beads
Diagram:

Take pictures of your beads for each phase of meiosis I and
II without crossing over. Include notes with your name, date and meiotic stage
on index cards in the pictures. Please use the lowest resolution possible so
that your file does not become too large to submit.

Insert pictures here:

Prophase I
Metaphase I
Anaphase I
Telophase I
Prophase II
Metaphase II
Anaphase II
Telophase I
Cytokinesis
Trial 2 – Meiotic Division with Crossing Over Beads Diagram:
Take pictures of your beads for each phase of meiosis I and
II with crossing over. Include notes
with your name, date and meiotic stage on index cards in the pictures. Please use the lowest resolution possible so
that your file does not become too large to submit.

Insert pictures here:

Prophase I
Metaphase I
Anaphase I
Telophase I
Prophase II
Metaphase II
Anaphase II
Telophase I
Cytokinesis

Post-Lab Questions

1. What is
the ploidy of the DNA at the end of meiosis I? What about at the end of meiosis
II?

2. How are
meiosis I and meiosis II different?

3. Why do
you use non-sister chromatids to demonstrate crossing over?

4. What
combinations of alleles could result from a crossover between BD and bd
chromosomes?

5. How many
chromosomes were present when meiosis I started?

6. How many
nuclei are present at the end of meiosis II? How many chromosomes are in each?

7. Identify
two ways that meiosis contributes to genetic recombination.

8. Why is it
necessary to reduce the number of chromosomes in gametes, but not in other
cells?

9. Blue
whales have 44 chromosomes in every cell. Determine how many chromosomes you would
expect to find in the following:

i. Sperm
Cell:

ii. Egg
Cell:

iii. Daughter
Cell from Mitosis:

iv. Daughter
Cell from Meiosis II:

10. Research
and find a disease that is caused by chromosomal mutations. When does the
mutation occur? What chromosomes are affected? What are the consequences?

11. Diagram
what would happen if sexual reproduction took place for four generations using
diploid (2n) cells.
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Experiment 2: The Importance of Cell Cycle Control
For each of the five abnormalities you find online, copy and
paste a picture of it (and be sure to cite the URL for the picture)you will
not be photographing your own results for this section of lab, because youre
doing your research online for the questions below.
Data Tables and Post-Lab Assessment
1. [paste in your
online picture and cite the URL]

2. [paste in your
online picture and cite the URL]

3. [paste in your
online picture and cite the URL]

4. [paste in your
online picture and cite the URL]

5. [paste in your online picture and cite the URL]

Post-Lab Questions
1. Record
your hypothesis from Step 1 in the Procedure section here.

2. What do
your results indicate about cell cycle control?

3. Suppose a
person developed a mutation in a somatic cell which diminishes the performance
of the bodys natural cell cycle control proteins. This mutation resulted in
cancer, but was effectively treated with a cocktail of cancer-fighting
techniques. Is it possible for this persons future children to inherit this
cancer-causing mutation? Be specific when you explain why or why not.

4. Why do cells which lack cell cycle control exhibit karyotypes which look physically different than cells with normal cell cycle.

5. What are
HeLa cells? Why are HeLa cells appropriate for this experiment?