1} When to analyze DNA vs RNA: In what type of situations/diseases is it more appropriate to analyze one’s DNA and when it is more appropriate to analyze RNA samples?
2} Poly-A tail and 5′ cap:In eukaryotic cells, RNA transcripts undergo some processing after transcription, such as addition of 5’ cap and poly-A tail. What is the general role of these two events? Why do you think it might be more important for eukaryotes to have these modification compared to prokaryotes?
3} Deleterious effects of point mutations:A point mutation in DNA sequence can sometimes completely alter protein structure/function leading to a disease. Can you think of an example of such a disease? Describe the specific DNA sequence change and what happens to the protein due to the change.
4} Point mutations with little effect:In contrast to the previous question, a point mutation in DNA sometimes have very little effect on protein function and human health. In what situations a point mutation has minimum effect?
5) Exonuclease?:What is the major role of exonuclease? What might happen if this function is lost from the cells
6} Regulation of DNA replication vs RNA transcription: In the lecture, we talked a little bit about the regulation of RNA transcription, but not much about DNA replication. What do you think is the major differences? (Think about when does a particular RNA is transcribed, vs when DNA is replicated. Are all the genes transcribed at the same time?)
7} DNA/RNA calculations
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