Viruses are obligate intracellular parasites so they depend on their host for their survival. They cannot be grown in non-living culture media or on agar plates alone, they require living cells to support their replication. For many years, growing viruses in the laboratory environment proved challenging and impossible for some strains. Read the following link for further information then address the questions below in your initial post.
The History of Vaccines. Accessed at Early Tissue and Cell Culture in Vaccine Development at http://www.historyofvaccines.org/content/articles/early-tissue-and-cell-culture-vaccine-development on September 16, 2016.
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