10. Answer the following questions, referring to the chart. Which tubes showed that starch or cellulose was still present? Which tubes tested positive for the presence of reducing sugars? How does the effect of freezing differ from the effect of boiling? CHART 2 Tube no. Enzyme Digestion of Starch and Cellulose 1 Amylase Starch pH 7.0 buffer Freeze first, then incubate at 37C for 60 minutes 2 Amylase Starch pH 7.0 buffer 37C 60 min 3 Amylase Glucose pH 7.0 buffer 37C 60 min 4 Amylase Cellulose pH 7.0 buffer 37C 60 min 5 Amylase Cellulose D.I. Note: D.I. Does amylase use cellulose as a substrate?