1. Explain your answers to the following.

A. The binding affinity between a peptide agonist and its specific G protein coupled receptor involves which types of chemical bonds?

B. The binding affinity between a peptide agonist and its specific G protein coupled receptor alone determines the sensitivity of a cell to the agonist peptide.

2. True or False. Explain your answer. G beta gamma subunits initiate MAPK cascade signaling via SH domains.

3. Describe how you would experimentally demonstrate the requirement for a G protien in agonist-stimlulated PLC activity

4. Describe how you would experimentally demonstrate that a specific G protein subunit beta is required for peptide agonist-stimulated increases in MAPK activity.

5. Describe how the GPCR and MAPK pathways ‘cross-talk’ or interact with each other.